Lymph-Node Resident CD8α+ Dendritic Cells Capture Antigens from Migratory Malaria Sporozoites and Induce CD8+ T Cell Responses
Figure 2
Sporozoite migration to the DLN is required for robust CD8+ T cell priming.
A. Naïve mice were injected ID with 2×104 P. berghei CS5M sporozoites treated for 20 minutes with 25 ug/ml of mAb, 2G3 (isotype control) or anti-P. berghei CS (3D11), or 16 ug/ml of Fab fragments prepared from the 3D11 antibody. Total RNA was isolated from DLNs at the indicated times. Parasite burdens were quantified by RT-PCR and pooled from 2 similar experiments; mean ± SEM, n = 10/group. B. 5×103 naïve OT-1 cells were transferred to mice. 24 hours later, mice were injected ID with 2×104 irradiated P. berghei CS5M sporozoites treated with 25 ug/ml 2G3 or 3D11 mAb or 16 ug/ml 3D11:Fab. Proportion of CD8+ T cells of OT-1 origin recovered 10 days post-inoculation; n = 5/group, mean ± SEM. Data representative of 2 similar experiments. C. 5×103 naïve OT-1 cells were transferred to mice. Mice were injected ID with 2×104 irradiated P. berghei CS5M or P. berghei CS5MΔN sporozoites 1 day after cell transfer. Proportion of CD8+ T cells of OT-1 origin recovered 10 days post-inoculation; n = 5/group, mean ± SEM. Data are representative of 4 similar experiments. D. 5×103 naive OT-1 cells were transferred to mice. 1 day after transfer mice were immunized IV with 2×104 irradiated P. berghei CS5M sporozoites or P. berghei CS5MΔN sporozoites. Expansion of OT-1 cells was measured 10 days after inoculation; mean ± SEM, n = 4–6/group. Data representative of 3 similar experiments.