Cell Cycle-Independent Phospho-Regulation of Fkh2 during Hyphal Growth Regulates Candida albicans Pathogenesis
Fig 9
Phosphorylation of Fkh2 does not alter its localisation, but affects its interaction with the chromatin modifier Pob3.
A) Fkh2-YFP/GFP localisation in log phase yeast cells or 40 min after hyphal induction. Images are taken at x100 magnification. Scale bar, 5 μm. B) Co-IP experiments of Fkh2 with Pob3 and Srp1 from samples taken 40 min post hyphal induction. 2 mg of total protein was used for IP and 50 μg for the lysates. IP products were washed twice with lysis buffer containing 150 mM NaCl. C) Interaction of Pob3 with Fkh2 phospho-mutants, performed as above. The co-immunoprecipitation was quantified by normalising the αHA signal (Pob3) against the immunoprecipitated αGFP (Fkh2) for each IP, and then expressing this value as a fraction of the wild type value.