Cell Cycle-Independent Phospho-Regulation of Fkh2 during Hyphal Growth Regulates Candida albicans Pathogenesis
Fig 5
GO analysis of fkh2(6A) and fkh2ΔΔ mutants.
A) Venn diagram showing the number of genes down regulated in the fkh2(6A) and fkh2ΔΔ mutants and the overlap in the two data sets. B) Genes significantly more down regulated in fkh2ΔΔ mutant compared to the fkh2(6A) mutant and genes more down regulated in the fkh2(6A) mutant compared to fkh2ΔΔ. Each panel shows the genes down regulated at least two fold at the 5% FDR threshold in fkh2(6A) or fkh2ΔΔ (empirical Bayes moderated t-test). Genes significantly down regulated in fkh2ΔΔ (above) or fkh2(6A) (below) are highlighted in the solid points. Those genes significantly (5% FDR, empirical Bayes moderated t-tests) more down regulated in fkh2ΔΔ than fkh2(6A) are shown in blue (top panel), or more down regulated in fkh2(6A) than fkh2ΔΔ are shown in red (lower panel). Right: GO pathways enriched in genes in each category highlighted on the left. Note for reasons explained in the text the figures in this panel are not consistent with the Venn diagram shown in panel A. C) qPCR comparing the expression of hyphal associated transcripts in the Fkh2 phosphorylation mutants. Expression levels are normalised against ADE2 and shown relative to the expression in the fkh2/FKH2 strain. Means are from two independent biological repeats, each with three technical replicates. Vertical bars are equal to one standard error.