Characterization of the Mycobacterial Acyl-CoA Carboxylase Holo Complexes Reveals Their Functional Expansion into Amino Acid Catabolism
Fig 5
Growth phenotype characterization and complementation of mycobacterial accD1 and accA1 genes.
(A) Growth of M. smegmatis wt and knockout strains in minimal 7H9 media supplemented with isovalerate (IVAL). The wt is in green; ΔaccD1-ΔaccA1 is in red; ΔaccD2-ΔaccA2 (control) is in blue. Error bars are in black. (B) Rescue of the M. smegmatis growth phenotype observed in isovalerate-containing minimal media by complementing ΔaccD1-ΔaccA1 (blue line) with the M. tuberculosis homolog of AccD1-AccA1 (red line). The wt is in green. Average values for the culture density were calculated from four replicates.