Characterization of the Mycobacterial Acyl-CoA Carboxylase Holo Complexes Reveals Their Functional Expansion into Amino Acid Catabolism
Fig 2
AccD1-AccA1 holo complex formation.
(A) SDS-PAGE showing the Ni-affinity eluate (lane 2), and after gel filtration (panel B) in lane 3; molecular markers are shown in Lane 1. (B) Size-exclusion chromatogram of purified AccD1-AccA1. The elution volume and molecular mass of the molecular weight calibration standards are indicated. The equation describing the linear regression line is f(x) = -0.16x + 4.6 and has a R2 = 0.99.