A Rho GDP Dissociation Inhibitor Produced by Apoptotic T-Cells Inhibits Growth of Mycobacterium tuberculosis
Figure 2
A subset of Foxp3+ cells express D4GDI.
(A) CD4+ cells from PBMC of 6 donors with LTBI were cultured with autologous monocytes and γ-irradiated M. tb (10 μg/ml). After 4 days, CD4+CD25+ (85–90% Foxp3+) and CD4+CD25‑ (<5% Foxp3+) cells were isolated, and cultured overnight in serum-free medium. Culture supernatants were subjected to Western blotting with anti-D4GDI. Data from 2 individuals with LTBI are shown. (B) CD4+ cells and autologous monocytes from 6 donors were cultured as in panel A. After 4 days, the percentages of D4GDI+ cells in CD4+Foxp3+ and CD4+Foxp3‑ cells were determined by flow cytometry. Mean values, p values and SEs are shown. (C) A representative flow cytometry plot is shown. (D) PBMC from 5 individuals with LTBI were cultured with γ-irradiated M. tb H37Rv. After 4 days, the percentages ofCD4+Foxp3+D4GDI+IFN-γ+, CD4+Foxp3+D4GDI+IL-10+ and CD4+ Foxp3+D4GDI+TGF-β+ cells were determined by flow cytometry. A representative flow cytometry plot is shown.