DNA Is an Antimicrobial Component of Neutrophil Extracellular Traps
Figure 2
Quantification of NETosis in human neutrophils ex vivo and mouse neutrophils during an infection.
(A) Human neutrophils were stimulated with PMA, P. aeruginosa, E. coli and S. aureus and Sytox green fluorescence was measured as an indicator of DNA release by NETosis after 1 hr stimulation. Exogenous DNase was added as a control to confirm extracellular DNA presence in NETs, indicated by a plus sign (+). Asterisks denote a significant difference in extracellular DNA release between stimulated and unstimulated neutrophils (white bar) (**P<0.01, ***P<0.001). Each value shown is an average from 6 replicates with error bars representing the standard error. (B) The total number of NETs in uninfected mice, or infected mice with P. aeruginosa PAO1 or S. aureus and (C) the total NET area quantified. # denotes a significant difference in NET area and number in P. aeruginosa PAO1 infected mice compared to the uninfected control (#P<0.05). Asterisks denote a significant difference in NET area and number in mice infected with P. aeruginosa compared to S. aureus infected mice (***P<0.001).