Crystal Structure of Calcium Binding Protein-5 from Entamoeba histolytica and Its Involvement in Initiation of Phagocytosis of Human Erythrocytes
Figure 5
Immunolocalization of EhCaBP5 in E. histolytica.
Trophozoites grown for 48 h were transferred to pre-warmed coverslips for 10 min at 37°C. The cells were then fixed with 3.7% paraformaldehde/PBS, permeabilized with 0.1% triton X-100/PBS and then stained with anti-EhCaBP5 antibody followed by secondary antibody Alexa 488. Hoechst was used to stain the nuclei. Bar represents 10 µm. Magnification 60X.