A Natural Genetic Variant of Granzyme B Confers Lethality to a Common Viral Infection
Figure 5
GzmBW/W mice are sensitive to infection with Δm157 MCMV.
(A) B6 mice (black square) or GzmBW/W mice (white square) were infected with 2×104 pfu of MCMV Δm157 and survival monitored over the indicated time course (n = 5 for each group). ***P<0.0001. (B) The indicated mouse strains were infected with 2×104 pfu of MCMV Δm157, the indicated organs were removed at day 7 post-infection, and viral load quantified by plaque assay. Data are pooled from 2 independent experiments, mean ± SEM are plotted, where n≥8. *P<0.05. (C) Livers from uninfected or MCMV-infected mice were isolated at day 6 post-infection, fixed and tissue sections stained with haematoxylin and eosin. The results are representative of two independent experiments. (D) Liver enzymes in the serum of B6 mice (black bar) or GzmBW/W mice (white bar) were measured at day 6 post-infection. Data are pooled from two independent experiments where n≥5. *P<0.05.