P47phox−/− Mice Are Compromised in Expansion and Activation of CD8+ T Cells and Susceptible to Trypanosoma cruzi Infection
Figure 6
The p47phox−/− mice lacked the ability to develop CD8+T cell response to T. cruzi infection.
Mice were infected with T. cruzi, and harvested at day 30 pi. Splenocytes were in vitro incubated for 48 h in presence or absence of TcL as 2nd antigenic stimulus (controls: concanavalin A). (A) Cell proliferation determined by an MTT assay. (B&C) After in vitro stimulation (±TcL), splenocytes from infected mice were labeled for 30-min with fluorescent- conjugated specific antibodies as described in Materials and Methods, and analyzed by flow cytometry. Splenic frequency of CD4+ and CD8+ T cells (B.a) are shown. Shown are also the mean percentage of CD8+ T cells that were Ki67+ (PerCPCy 5.5, B.b), IFN-γ+ (e-Fluor, B.c), and CD107a+ (PerCPA, B.d). Representative quandrant images of flow cytometry analysis of T cell subsets are shown in (C).