P47phox−/− Mice Are Compromised in Expansion and Activation of CD8+ T Cells and Susceptible to Trypanosoma cruzi Infection
Figure 2
T. cruzi uptake, intracellular replication and release by p47phox−/− phagocytes.
(A) Bone marrow (BM) and splenic cells were isolated from WT and p47phox−/− mice and in vitro incubated for 24 h with CFSE-labeled T. cruzi followed by 30 min with fluorescence conjugated anti-CD11b (macrophage marker) and anti-Ly6G (neutrophil marker) antibodies. The antibody-labeled cells were fixed, and analyzed by flow cytometry. Representative flow cytometry analyses of BM cells infected with CFSE-labeled T. cruzi are shown. (B) Bar graphs of the percentage of BM (Ba) and splenic (Bb) cell populations (CFSE+CD11b+: infected macrophages, CFSE+Ly6+: infected neutrophils). Data are derived from 3 independent experiments. (C) Supernatants of infected splenic monocytes were monitored for parasite release at 24 h and 48 h post-incubation by light microscopy. Data are presented as mean ± SD and significance presented as *normal-versus-infected, #WT/infected-versus-p47phox−/−/infected (*, #p<0.05, **,##p<0.01.