Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent Manner
Figure 9
MHV-S2′FCS fuses in early endosomes.
siRNA-mediated gene silencing was performed as described in the legend to Figure 1. At 72 h post transfection, HeLa-mCC1a were inoculated with MHV-EFLM or MHV-S2′FCS at MOI = 0.2 and incubated until 7 hpi. Infection levels were determined by measuring the luciferase activity in cell lysates relative to mock-treated cells. Dotted line shows the lower 95% confidence interval of the negative siRNA controls. Error bars represent SEM, n = 3*3.