Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent Manner
Figure 6
Inhibition of lysosomal proteases prevents MHV fusion.
The MHV fusion assay was performed on HeLa-mCC1a-ΔM15 cells as described in the legend to Figure 3, in the presence of the protease inhibitors CPI, AEBSF, Aprotinin, Leupeptin, Pepstatin A, Camostat, and Phosphoramidon. As controls, cells were treated with solvent DMSO, MHV fusion inhibitor HR2 peptide (HR2), and lysosomotropic agent ammonium chloride (NH4Cl). Fusion was determined relative to the number of FIC-positive cells after mock treatment (UNTR). Error bars represent SEM, n = 3.