Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent Manner
Figure 5
MHV infection depends on endosomal maturation.
A) HeLa-mCC1a cells were pretreated with increasing concentrations of Bafilomycin A1 (BafA1) for 30 min and subsequently infected with luciferase expressing MHV, VSV, or IAV in the presence of BafA1. Infection levels were determined by assaying the luciferase activity in cell lysates relative to lysates of infected cells that had been mock treated. Error bars represent SEM, n = 3*3. B) Haploid cells (HAP1), haploid cells lacking VPS33A (H1-ΔV33) or VPS33A-lacking haploid cells retransfected with FLAG-tagged VLP33A (H1-ΔV33-fV33) were infected with luciferase expressing MHV, VSV, or IAV. Cells were lysed at 7 h (MHV and VSV) or 16 h post infection. Infection is displayed relative to virus-driven luciferase expression levels in HAP1 cells. Error bars represent SEM, n = 3*3.