Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent Manner
Figure 2
Endocytosis affecting agents indicate clathrin-mediated endocytosis and endosome maturation to be important in MHV infection.
HeLa-mCC1a cells, inoculated with MHV-EGFPM at MOI = 0.5, were treated with the different inhibitors from 30 min prior to 8 h post inoculation (0–8 h) or from 2–8 h post inoculation (2–8 h; hatched bars): ammonium chloride (NH4Cl), Bafilomycin A1 (BafA1), Chloroquine (Chloq), Chlorpromazine (Chlopro), Monensin (Mon), Dynasore, Dyngo-4A, EIPA, Latrunculin A (LatA), Jasplakinolide (Jasp), Cytochalasin B (CytoB), Cytochalasin D (DytoD), Nocodazole (Noc), MG132, Brefeldin A (BrefA), as well as solvents dimethyl sulfoxide (DMSO) and methanol (MeOH). Infection was determined by FACS and displayed relative to the infection level observed in mock-treated cells (UNTR). Error bars represent SEM, n = 3.