The Plasmodesmal Protein PDLP1 Localises to Haustoria-Associated Membranes during Downy Mildew Infection and Regulates Callose Deposition
Figure 5
PDLP1 is required for callose deposition in haustorial encasement.
(A) Aniline blue staining of callose in Col-0, PDLP OE, pdlp1,2,3 and pmr4 mutant leaves 5 DPI with Hpa Noco2 identifies that pdlp1,2,3 produces fewer encased haustoria at this stage of infection, similar to the callose synthesis mutant pmr4. Quantification (B) of stained haustoria confirms that pdlp1,2,3 produces significantly fewer aniline blue stained encasements than Col-0. PDLP1 OE plants produce more stained encasements than Col-0 plants per field of view. Error bars represent the standard error of the mean. * p-value <0.05, *** p-value <0.001 by Student's t-test. (C) At higher magnification, aniline blue stained encasements in PDLP1 OE cells appear thicker than Col-0 encasements. The transmitted light image of a pdlp1,2,3 haustorium suggests that there is some encasement (arrow) of the haustorium but this structure does not stain with aniline blue. Asterisks indicate haustoria. Scale bars are 100 µm (A) and 10 µm (C).