The Plasmodesmal Protein PDLP1 Localises to Haustoria-Associated Membranes during Downy Mildew Infection and Regulates Callose Deposition
Figure 2
PDLPs localise to the extra-haustorial membrane.
PDLP1-GFP is observed at the EHM prior to encasement. (A) In unencased haustoria, or those with a developing encasement at the haustorial neck, PDLP1-GFP is present in the EHM surround the haustorium (n = 50/50). PD-located signal is indicated with solid arrows, while open arrows indicate signal associated with haustoria. (B) As the encasement develops (stained with aniline blue, open arrows) and surrounds the entire haustorium PDLP1-GFP fluorescence remains associated with the haustorium and PDLP1-GFP positive bodies can be seen at the encasement periphery (see E). (C) When encasements are mature PDLP1-GFP is no longer associated with the structure. (D) Scheme of the development of the Hpa haustorial encasement, stages I, II and III are indicated in (A–C) for reference. (E) Enlargement of developing encasements shows PDLP1-GFP positive bodies at the periphery of the encasement (arrows). Scale bars are 20 µm (A–C) and 10 µm (E).