The Pseudomonas aeruginosa Type III Translocon Is Required for Biofilm Formation at the Epithelial Barrier
Figure 5
Supernatants from PAK-infected cells restore cell-associated aggregation and enhance abiotic biofilm formation.
(A) Cell-associated aggregation by PAKΔpopB in the presence of various supernatants was quantified using spinning disk confocal microscopy using the same method as in Figure 2D (n≥3 independent experiments). Data are mean ± SEM. ***p<0.001 compared to PAK. (B) PAK and isogenic T3SS mutants were inoculated into tissue-culture media (MEM) or supernatant from PAK-infected cells and assessed for biofilm formation on microtiter plates (n≥3 independent experiments). Data are mean ± SEM. *p<0.05 compared to control in MEM. Statistics in Supplemental Statistical Analysis (Text S1).