Antimicrobials, Stress and Mutagenesis
Figure 2
Antimicrobial peptides do not elicit the SOS (top panel) or the rpoS pathway (bottom panel).
Induction of the SOSresponse or rpoS response is shown by a fluorescent ring around the zone of clearance (green for recA::GFP, blue for rpoS::β-gal at the edge of the inhibition zone). Positive controls with antibiotics (Cip: Ciprofloxacin, Ap: Ampicillin) are shown on the left. The bright central disc is a filter paper as source of the antibiotic/antimicrobial [22]. The amount of animicrobials on paper disks consisted of 50 µg of ampicillin, 5 µg of ciprofloxacine, 50 µg of cecropin A (Cec-A), 256 µg of human lysozyme (h-Lys), 50 µg of LL-37, 20 µg of Melittin, 200 µg of magainin II, and 20 µg of pexiganan.