Expression Profiling during Arabidopsis/Downy Mildew Interaction Reveals a Highly-Expressed Effector That Attenuates Responses to Salicylic Acid
Figure 2
Expression pattern of Hpa predicted effectors and potential cis-regulatory elements in Hpa.
(A) Expression pattern of predicted effectors expressed in at least one of three infections (1, 3, and 5 dpi) with Hpa Waco9. Expression levels were represented as TPM (tags per million) of total reads mapped to Hpa genome. Red lines indicate predicted effectors induced more than two fold at 3 dpi compared to the expression level in conidiospores (cs). Single and double asterisks indicate expression pattern of HaRxL76 and HaRxL62, respectively. A right line chart is magnification of left one. (B) Average expression pattern of genes in the indicated groups during the infection with Hpa Waco9. The induction levels compared to the level in cs were indicated by value of log2. (C to K) Distribution of motifs in coexpressed genes of Hpa and P. infestans. Nucleotide conservation of (C) the INR-FPR motif in “induced effectors”, (F) Motif I in “induced effectors” and (I) Motif II in “non-induced effectors” is displayed as sequence logos, based on hits within 200 nt (INR-FPR) and 500 nt (Motif I and II) upstream of the start codon. Bar charts indicate the percent of promoters within each group that contain (D, E) the INR-FPR motif within 200 nt and (G, H) Motif I and (J, K) Motif II within 500 nt upstream of the start codon. The analysis was done in promoters from (D, G, J) Hpa and (E, H, K) P. infestans. Asterisks indicate statistically significant over-representation of the motifs compared to population in “all genes” (p<1e-4), which is shown in Table S4.