Characterization of Uncultivable Bat Influenza Virus Using a Replicative Synthetic Virus
Figure 5
Pathogenicity of Bat-NS1 mutants in mice.
(A) Virus titers of Bat09:mH1mN1ss and NS1 mutants in mouse lungs at 3 and 5 dpi. Each mouse was intranasally inoculated with 104 TCID50 of each virus. (B) Mouse weight on each day post inoculation was represented as a percentage of the weight on day 0 (100%). Each mouse was intranasally inoculated with 105 TCID50 of the indicated viruses. (C) Survival rate of the mice inoculated with 105 TCID50 of the viruses. Higher virus doses were used in this experiment based on the PR8-NS1-126 control virus, which caused significant weight loss but had low mortality at 105 TCID50 so the attenuation of Bat-NS1 truncated viruses can be appropriately compared to it. *, P<0.05, truncated Bat09:mH1mN1ss-128 and Bat09:mH1mN1ss-73 compared to PR8-NS1-126.