Age-Dependent Enterocyte Invasion and Microcolony Formation by Salmonella
Figure 8
Mucosal tissue response following S. Typhimurium infection in neonate mice.
(A) Flow cytometric analysis of lamina propria myeloid immune cells prepared from 1-day-old neonates infected for 4 days with 102 CFU S. Typhimurium or non-infected age-matched control animals. Representative images are shown. (B) Quantitative analysis of CD11b+ F4/80+ MHCII− CD11c− tissue invading macrophages and CD11b+ F4/80− MHCII− CD11c− polymorphonuclear cells relative to total CD45+ cells in the lamina propria of 1-day-old neonates infected for 4 days with 102 CFU S. Typhimurium or non-infected age-matched control animals. The results represent the mean ± SD from 2 independent experiments (n = 4). (C) Hematoxilin and eosin staining of intestinal tissue sections of neonatal mice (small intestine) left untreated or 4 day p.i. with S. Typhimurium (102 CFU). Bar = 50 µm.