Reorganization of the Endosomal System in Salmonella-Infected Cells: The Ultrastructure of Salmonella-Induced Tubular Compartments
Figure 2
Double membrane SIT contain cytoskeletal filaments and ribosomes.
HeLa cells were infected with S. enterica WT as for Figure 1. A), B), C) and D) show examples of double-membrane SIT with microtubules, indicated by dark blue arrowheads. Orange and yellow arrowheads indicate inner and outer membranes of SIT, respectively. Measurements of microtubules as shown in i) revealed an average diameter of 22 nm±2.3 nm. Higher magnifications are shown in panel ii to vii. E) shows an example of a double-membrane SIT containing thinner, actin-like filaments (light blue arrowheads). Note that the inner space of all double-membrane SIT (A–E) also contains ribosomes. Scale bars: 500 nm.