Incomplete Deletion of IL-4Rα by LysMCre Reveals Distinct Subsets of M2 Macrophages Controlling Inflammation and Fibrosis in Chronic Schistosomiasis
Figure 6
Lyz2lo macrophages develop features of AAMs in response to S. mansoni eggs.
IL-4Rαflox/ΔLysMCre mice (open bars) and littermate controls (solid bars) were left untreated (naïve), challenged with 5000 S. mansoni eggs i.p. 4 days before harvest (1o), 18 days before harvest (1o-rested), or challenged on both 18 days and 4 days before harvest (1o-rechallenged). A. Total peritoneal cells were sorted for F4/80hi CD11bhi cells at a purity of >90%. B. Representative 20× images of sorted F4/80hi CD11bhi macrophages after cytospin and hematoylin and eosin staining. C,D. The sorted cells were assayed for Il4rα and Lyz2 gene expression (C), and gene expression of markers of alternative activation (D). Fold change in gene expression is shown relative to the expression levels in sorted F4/80hi CD11bhi cells from naïve littermate controls. E. Surface expression of mannose receptor measured by flow cytometry on unsorted F4/80hi CD11bhi peritoneal cells from the same treatment groups. F. Arginase activity in sorted macrophages. (n = 3–6, ns = not significant) Data shown are mean ±SEM and represent at least two independent experiments.