Sialylation of Prion Protein Controls the Rate of Prion Amplification, the Cross-Species Barrier, the Ratio of PrPSc Glycoform and Prion Infectivity
Figure 5
2D analysis of brain- and spleen-derived PrPC.
2D analysis of Syrian hamster spleen (A) and brain homogenates (B). Diglycosylated and monoglycosylated full-length PrPC (FL) are marked by black and white triangles, respectively. Di-, mono- and unglycosylated C2 fragments (C2) in spleen or C1 fragments (C1) in brain are marked by bold, medium and thin arrows, respectively. PrPC, C1 or C2 dimers (D) are encircled. M stands for a marker lane: brain or spleen samples were diluted 10-fold and used as references for 2D gels. Blots were stained with SAF-84 antibody.