Structural Correlates of Rotavirus Cell Entry
Figure 8
Whole-cell cryo-tomography of surface-bound rotavirus particles.
A–C. Tomographic slices through individual virions, free (A) and bound (B,C) to cell membrane. Note differences in spike extension in B and C (red arrowheads). D–I. Tomographic slices (D–F) and isosurface renderings (G–I) of subtomogram averages of rotavirus particles. Subtomogram averages: (D,G & F,I) after applying icosahedral symmetry (D,G: 1080 repeats from 18 virions; F,I: 420 repeats from 7 virions); for (E,H), we classified the repeats and averaged only those 78 repeats from 5 virions for which spikes were in contact with membrane (blue density).