Human Cytomegalovirus pUL79 Is an Elongation Factor of RNA Polymerase II for Viral Gene Transcription
Figure 5
pUL79 does not alter a particular phosphorylated form of the RNAP II CTD domain.
HFF cells were infected with ADddUL79 at an MOI of 3 in the presence or absence of 1 µM Shld1. Cell extracts were harvested at 72 hpi and analyzed by ChIP assays. Rabbit antibody to pSer2 CTD, rat antibody to pSer5-CTD, and mouse antibody to non-phosphorylated CTD (8WG16) were used in ChIP assays. Normal rabbit, rat, and mouse IgGs were included as controls for non-specific precipitation, respectively. Immunoprecipitated DNAs were analyzed as described in Fig. 4B and the output-to-input DNA ratios are presented in (A). In addition, the immunoprecipitated amount of each phosphor-isoform of RNAP II CTD relative to that of total RNAP II (immunoprecipitated with antibody N-20) was also calculated and presented in (B). Data from four independent experiments were collected with standard deviations calculated by Prism 6 software. Statistical analysis was performed using Student's t test (*, P<0.05; **, P<0.01; ***, P<0.005).