Rabies Virus Hijacks and Accelerates the p75NTR Retrograde Axonal Transport Machinery
Figure 7
RABV is retrogradely transported with neurotrophin receptors.
(A–D), Retrograde transport of EGFP-RABV, added to the distal axon compartment of DRG explant previously treated with fluorescent antibodies against p75NTR and TrkA. Arrowheads: RABV puncta positive for p75NTR, arrows: RABV puncta positive for p75NTR and TrkA. Scale bar = 10 µm. (E,F) Co-localisation of RABV with p75NTR and TrkA calculated from two and one experiments, respectively. (G) Trajectories of RABV trafficked with neurotrophin receptors (NTFR, Blue) or without (Red), illustrating a more processive displacement over time of RABV with NTFR. (H) Merged kymographs of RABV (green) p75NTR (red) and TrkA (cyan), drawn for multi-channel time lapse. Vertical scale bar = 5 µm, horizontal scale bar = 40 seconds.