Rabies Virus Hijacks and Accelerates the p75NTR Retrograde Axonal Transport Machinery
Figure 6
RABV is transported in acidic compartments in sensory axons.
(A–D), Multi-channel live imaging of EGFP-RABV retrograde transport in DRG axons, along with the fluorescent cellular markers Lysotracker Red and Mitotracker Deep Red. Arrowheads: RABV particles positive for Lysotracker Red. Scale bar = 10 µm. (E) 75.9% of RABV puncta are co-localized with Lysotracker Red, thus found in acidic compartments along the axon, as opposed to 23.6% co-localization with random Lysotracker frames from the same time lapse (n = 3 separate experiments). (F) RABV does not co-localize with Mitotracker. (G–I) Kymographs of EGFP-RABV (G) and Lysotracker (H) are overlaid in (I) show kinetics of RABV (green) and Lysotracker (red) retrograde transport in DRG axons. Arrowheads: acidic transport of RABV. Horizontal scale bar = 5 µm, vertical scale bar = 40 seconds. *p<0.0005. n.s = non significant.