Capsid Protein VP4 of Human Rhinovirus Induces Membrane Permeability by the Formation of a Size-Selective Multimeric Pore
Figure 6
VP4 multimeric structure visualized by electron microscopy.
A. VP4GST was reconstituted in the membrane mimetic detergent DPC, applied to carbon coated grids and stained with uranyl formate. Scale bar = 20 nm. B. Individual ring-like structures were manually selected and cropped from unprocessed digital images. Scale bar = 10 nm. C. Class average images of particles automatically picked from a large image data set and classified into 7 classes. Scale bar = 5 nm. D. Class averages 3 and 7 (top-down views) analyzed by rotational harmonic analysis.