Israeli Acute Paralysis Virus: Epidemiology, Pathogenesis and Implications for Honey Bee Health
Figure 2
Relative abundance of negative strand RNA of IAPV genome copies in different tissues of honey bees and in situ hybridization analysis of queen somatic and germ tissues.
(A) The hemolymph harbored the minimal level of IAPV and therefore was chosen as a calibrator. The concentration of negative strand RNA of IAPV in other tissues was compared with the calibrator and expressed as n-fold change. The y-axis depicts fold change relative to the calibrator. (B) The slides were not hybridized with DIG-labeled IAPV probe (top row, negative control) and the slides were hybridized with DIG-labeled IAPV probe (bottom row). Positive signal is dark blue to purple and the negative areas are pink in color. The infected tissues of queen gut, ovary, spermatheca and queen eggs are indicated by a dark blue/purple color.