Lytic Gene Expression Is Frequent in HSV-1 Latent Infection and Correlates with the Engagement of a Cell-Intrinsic Transcriptional Response
Figure 6
Increasing viral activity is matched by progressive host neuronal transcriptional response.
(A) Violin plot representation of selected cellular gene expressions in LAT+Ntrk1+YFP+ neurons categorized based on their lytic gene expression profile. Log2Ex represents expression threshold (Et). Numbers in brackets show the number of individual cells analyzed. * both proportion (p<0.05) and expression levels (p<0.0167) are significant, ‡ only expression levels significant, # proportion and expression levels are not significant when comparing full-lytic to partial- and non-lytic subsets. (B) k-means clustering of Kendall tau rank correlation coefficients (τ) of every pair of 48 genes from single LAT+Ntrk1+YFP+ neurons. Correlation coefficient matrix of non-lytic neurons was clustered according to the optimal clustering observed in full-lytic neurons. (C) Complete-linkage clustering of Kendall tau rank correlation coefficients (τ) between expression profiles of every pair of 48 genes from single LAT+Ntrk1+YFP+ neurons. Correlation coefficient matrices of non-lytic and full-lytic neurons were independently clustered. All data are from the main single cell gene expression dataset.