Lytic Gene Expression Is Frequent in HSV-1 Latent Infection and Correlates with the Engagement of a Cell-Intrinsic Transcriptional Response
Figure 3
Latent HSV resides in a subpopulation of sensory neurons.
(A) YFP+ and YFP− cells were identified and captured from DRG sections of infected ROSA-YFP mice. (B) DRG sections from KOS0152- or WT HSV-infected ROSA-YFP mice (day 20) were stained for Nissl bodies and examined under the confocal microscope. Arrows identify HSV-infected neurons. (C) Copy number of viral DNA in individual YFP+ cells (day 15) captured by LCM is shown in a histogram. (D) Number and percent of Ntrk1+ and Ntrk1− neurons from populations of infected (YFP+) and uninfected (YFP− and uninfected DRG) neurons in the main set of single cell expression data are shown in pie charts. Data in (A and C) are pooled from two independent experiments. Images in (B) are from one experiment. (D) As for all single cell gene expression data, the neuron sections analysed were combined from 4 independent infections (YFP+ and YFP− neurons) or 3 independent experiments (neurons from uninfected DRG). Numbers in brackets show the number of individual cells analyzed.