Lytic Gene Expression Is Frequent in HSV-1 Latent Infection and Correlates with the Engagement of a Cell-Intrinsic Transcriptional Response
Figure 2
The number of infected cells remains stable during latency.
(A) Number of infected (β-galactosidase+) cells from KOS/pCMV/eGC infected DRG at the times shown post inoculation. (B) DRG explants from ROSA-YFP mice (day 20) infected with KOS0152 or WT HSV, or uninfected mice were examined under the 2 photon intra-vital microscope. Arrows identify HSV-infected cells. (C) DRG sections from KOS0152- or WT HSV-infected ROSA-YFP mice (day 50) were examined under the laser capture microscope for YFP expression and Hoechst nuclear stain. Data in (A) are pooled from two independent experiments and represented as mean ± S.E.M. Images in (B and C) are from two independent experiments.