IscR Is Essential for Yersinia pseudotuberculosis Type III Secretion and Virulence
Figure 5
Deletion of IscR leads to increased transcription of Fe-S cluster biogenesis genes and robust transcription of T3SS genes.
Quantitative real-time PCR analysis of WT and ΔiscR Y. pseudotuberculosis was performed (A) for the Fe-S cluster biogenesis genes, iscS and erpA and (B) for the T3SS genes, yscF, yscN and lcrF. Experiments were carried out from cultures grown in M9 at 37°C for 3 h. Shown are the averages ± SEM from three independent experiments. *p<0.05, **p<0.001, ***p<0.0001 as determined by a Student t test.