Tetherin Can Restrict Cell-Free and Cell-Cell Transmission of HIV from Primary Macrophages to T Cells
Figure 5
Tetherin retains HIV on infected MDMs.
(A) MDMs were infected with R3A-(+), -(−), or -Udel for seven days, lysed, and analysed by western blotting. Note that the γ-adaptin blot from this donor is shown in Fig. 3B. The p24 Gag band intensities were quantified and normalised to the p55 Gag levels. Bars represent the means ± SD of duplicate samples from three donors relative to R3A-(+) (set at 1). (B) MDMs were infected with R3A and transfected with control or Tetherin siRNA the next day. Cells were lysed six days later and analysed by western blotting. (C) p24 Gag concentrations in cell-free culture supernatants from R3A-infected MDMs were determined using ELISAs. Bars represent the means ± SD of triplicate samples from three donors relative to R3A-(+) (set at 100%). p values were calculated using a paired Student's t-test before normalisation. (D) p24 Gag concentrations in cell-free culture supernatants from R3A-infected, siRNA-treated MDMs were determined using ELISAs. Bars represent the means ± SD of triplicate samples from a representative experiment, relative to control RNAi/R3A-(+) (set at 100%).