Large Scale RNAi Reveals the Requirement of Nuclear Envelope Breakdown for Nuclear Import of Human Papillomaviruses
Figure 5
vDNA and L2-GFP associate with mitotic chromosomes.
(A) HeLa Kyoto cells were infected with EdU-HPV16. Cells were stained for host DNA and EdU to detect the vDNA after fixation at 20 h p.i.. Depicted are host cell chromatin (left), the vDNA (middle), and merge (right) of confocal sections of an aphidicolin-treated cell (G1/S) and untreated mitotic cells. Note that vDNA commenced association with chromosomes upon entry into mitosis. Indicated are the mitotic stages based on chromosomal organization. (B) as in (A). Quantification of vDNA localization to host chromatin ± SD (n = 5). (C) HeLa H2B-mCherry cells were transiently transfected with an L2-GFP expression plasmid. Cells were imaged by video microscopy 32 h post transfection for 24 h in 5 min intervals. Depicted are images of a L2-GFP expressing cell progressing through mitosis (from Video S9). (D) Timing of L2-GFP chromosomal association relative to metaphase plate formation (n = 88).