Contribution of Specific Residues of the β-Solenoid Fold to HET-s Prion Function, Amyloid Structure and Stability
Figure 2
Effect of alanine mutations on [Het-s] activity in vivo.
Δhet-s strain was transformed with the given mutants and the fraction of transformants producing a barrage reaction to [Het-S] (dark grey) and able to convert a [Het-s*] to the prion phenotype (light grey) is given in %. The mutants were grouped into three functional groups: mutants with wild-type or close to wild-type activity (>60%) were labeled by a green dot, slightly affected mutants (>30, <60%) were labeled by an orange dot, and strongly affected mutants (<30%) by a red dot, respectively. The “*” sign indicates that no transformant displayed the given activity.