Suppressor of Cytokine Signaling 4 (SOCS4) Protects against Severe Cytokine Storm and Enhances Viral Clearance during Influenza Infection
Figure 5
Defective TCR responses in Socs4R108X/R108X CD8 T cells.
CD4 or CD8 T cells were purified from Socs4R108X/R108X or wild-type spleens by negative selection, plated onto anti-CD3 coated plates in the presence of IL-2 and analysed at the indicated timepoints. (A) Q-PCR analysis showing increased expression of Socs4 mRNA in purified T cells from Balb/c mice following TCR engagement (mean±SEM, n = 3). (B) Cells were analysed by flow cytometry for the expression of the T cell activation marker, CD62L (mean±S.D.), n = cells derived from 3 mice. (C–D) Cells were labelled with Cell Trace Violet (CTV) for analysis of proliferative responses. Cell numbers per division are shown in the left hand panels; total cell numbers in the centre panels, (C) CD4 T cells, (D) CD8 T cells. Results are shown as the mean±S.E.M. using pooled data from 2 independent experiments (n = 5 mice per group per experiment). Representative histograms are plotted for each subset (day 4; right hand panels).