Dual-Site Phosphorylation of the Control of Virulence Regulator Impacts Group A Streptococcal Global Gene Expression and Pathogenesis
Figure 5
RNA-Seq analysis of various GAS strains.
Six strains were grown in quadruplicate to mid-exponential phase. (A) Heat-map of log2 transcript levels of selected genes for the indicated strains relative to strain MGAS10870. Color value scheme is shown below the figure. (B) Pairwise comparison of log2 transcript values relative to the wild-type strain MGAS10870 for the indicated strains. Each dot represents a particular gene. Values for strain 10870ΔcovR are plotted along the X-axis whereas values for strain CovR-D53A (red) and CovR-T65E (blue) are plotted along the Y-axis. The dotted lines indicate the 1.5-fold difference which was on e of our criteria for statistically significant transcript levels. (C) Transcript level data relative to strain MGAS10870 are plotted as in panel A with values for strain 10870ΔcovR plotted along with X-axis and values for strain 10870ΔcovS (red dots) and CovR-T65A (blue dots) plotted along the Y-axis.