Deficient IFN Signaling by Myeloid Cells Leads to MAVS-Dependent Virus-Induced Sepsis
Figure 5
Serum cytokine levels in WNV-infected mice.
A. Ifnar−/−, CD11c Cre+ Ifnarf/f, LysM Cre+ Ifnarf/f, and Cre− Ifnarf/f mice (n = 13 for each group) were infected 102 PFU of WNV. 48 hours later, serum was collected and the concentration of IL-1ß, IL-6, and TNF-α was determined. Mean values ± SD are shown. B. Forty-eight hours after WNV infection splenic CD11c+ cells were isolated from Ifnar−/−, CD11c Cre+ Ifnarf/f, and Cre− Ifnarf/f mice by positive selection with antibody-coated magnetic beads (n = 3 for each group). Total RNA was prepared and qRT-PCR was used to determine the amount of IL-1ß, IL-6, TNF-α, and WNV E RNA. The mean values ± SD are shown. The data is expressed as fold-increase in mRNA relative to samples from WNV-infected Cre− Ifnarf/f mice.