Kind Discrimination and Competitive Exclusion Mediated by Contact-Dependent Growth Inhibition Systems Shape Biofilm Community Structure
Figure 6
Analysis of Bt-Bp chimeric strains early in biofilm development.
Confocal microscopy of early time points in biofilm development of BtE264, Bt-Bp1106a-1, Bt-Bp1106a-2, and BtΔbcpAIOB bacteria, each constitutively expressing gfp. A) and B) Images taken at 6 and 24 hours in the plane of focus at the bottom of the biofilm (i.e., of bacteria directly attached to the glass bottom biofilm chambers). White scale bar = 10 µm. C) Fluorescent and DIC merged images taken at 24 hours in a plane of focus approximately half way between the top and bottom of the biofilm. Arrows indicate pillar structures. Black scale bar = 32 µm.