The Apical Complex Provides a Regulated Gateway for Secretion of Invasion Factors in Toxoplasma
Figure 2
3D-SIM of RNG2 location relative apical polar ring marker RNG1 and conoid marker CAM1.
(A-C) RNG1-GFP (pseudo-colored blue), and (D-F) CAM1-GFP (pseudo-colored blue) colocalized with HA (green) and cMyc (red) of the HA-RNG2-cMyc fusion. Conoid position is indicated. Individual immuno-signals are shown in monochrome, and colored in the merged image where GAP45 labeling is shown by a dashed outline. Right hand column shows GAP45 labeling of each cell at 0.25 magnification. Scale bars = 500 nm.