Alveolar Macrophages Are Essential for Protection from Respiratory Failure and Associated Morbidity following Influenza Virus Infection
Figure 6
Increased morbidity and respiratory failure in mice upon selective depletion of alveolar macrophages prior to pulmonary virus infection.
(A–E) Mice were treated with clodronate or control PBS liposomes 2 days prior to infection with 50 pfu PR8 influenza virus. Loss of body weight (A) and temperature (B) was monitored. (C) Survival was assessed over a period of 3 weeks after infection. Values indicate mean ± SEM of 6 mice per group. Arterial blood oxygen saturation (D) and O2 partial pressure (E) was measured in infected animals at d8. (F–H) WT and Csf2−/− mice were infected with 104 pfu vaccinia virus WR i.t. Loss of body weight (F), loss of temperature (G) and survival (H) was monitored. Values indicate mean ± SEM of 7–8 mice per group.