Alveolar Macrophages Are Essential for Protection from Respiratory Failure and Associated Morbidity following Influenza Virus Infection
Figure 2
GM-CSF promotes development of CD103+ DCs and expression of CD103 in young and adult mice, respectively.
Analysis of lung DC subsets in (A–C) 6–8 week-old and (D–E) 6-day-old mice. (A, D) Gated cells in dot plots show CD103+ and CD11b+ DCs with values indicating percentages among CD11c+Siglec-F− lung DCs. Cells were pre-gated on CD45+eFluor780− viable cells. (B,E) Bar graphs depict total number of CD103+ and CD11b+ DCs in the lung. (C) Mean fluorescence intensity (MFI) of CD103 expression on CD103+CD11b− DCs. Values shown represent the mean ± SD (n = 3–4). (F) Analysis of lung DC subsets in mixed BM chimeras generated by transfer of 1∶1 mixture of CD45.1+WT∶CD45.2+Csf2rb−/− or control CD45.1+WT∶CD45.2+WT into lethally irradiated recipients. Bar graphs show the percentage of CD45.2+WT and CD45.2+Csf2rb−/− cells among CD103+ and CD11b+ DCs in the lung. The mean ± SD is shown (n = 3).