Fungal Chitin Dampens Inflammation through IL-10 Induction Mediated by NOD2 and TLR9 Activation
Figure 5
Chitin co-localisation with NOD2, TLR9 and MR.
Confocal fluorescence microscopy of mBMMφs from wild type mice stimulated with chitin for 20 min. NOD2 protein was detected with anti-mouse NOD2 antibody, TLR9 with anti-mouse TLR9 antibody, MR with anti-mouse CD206-antibody and chitin was detected using a chitin-binding reporter construct (ChBD-HuCκ). Images show chitin co-localisation with NOD2 (A and C), TLR9 (A and B) and MR (B and C). Images are representative of two independent experiments, scale bars = 10 µm.