Fungal Chitin Dampens Inflammation through IL-10 Induction Mediated by NOD2 and TLR9 Activation
Figure 4
Chitin induced IL-10 secretion requires mannose receptor interaction but not uptake.
(A) Chitin was incubated with liposomal transfection reagent DOTAP for 15 min at room-temperature before used to stimulate mBMMφs from wild type, TLR2, TLR9, NOD2, MR and dectin-1-deficient mice for 24 h or (A and B) mBMMφs were treated with CytD for 1 h prior chitin stimulation. Values represent mean ± SEM, n = 4, *p<0.05, **p<0.01.