IL-1α and Complement Cooperate in Triggering Local Neutrophilic Inflammation in Response to Adenovirus and Eliminating Virus-Containing Cells
Figure 5
The recruitment of Ly-6G+7/4+ leukocytes to the splenic marginal zone partially depends on functional IL-1α-IL-1RI signaling, but not on IL-1β.
(A) Splenocytes were harvested from WT, Il1a−/−, Il1b−/−, Il1a/b−/−, and Il1r1−/− mice injected with Ad 2 hours after the virus challenge. Mock – WT mice were injected with saline. N = 5. * - P<0.05. n.s. – not statistically significant, between indicated experimental groups. Error bars represent standard deviation of a mean. (B) Distribution of Ly-6G+ leukocytes on sections of the spleens harvested from WT, Il1a−/−, and Il1a/b−/− mice injected with Ad virus (HAdv5). Mock – mice were injected with saline. The MZ is outlined by the dotted lines. Spleen sections were simultaneously stained with FITC anti-Ly-6G (green) and anti-Ad-hexon (red) antibodies and pictures were taken using a Leica fluorescent microscope. Representative spleen sections for each experimental setting are shown. N = 5. (C) Quantitative representation of Ly-6G+ (PMN) distribution on sections of the spleens of mice injected with Ad 2 hours after the virus administration. The quantification of Ly-6G+ cell distribution was done as described in Figure 3B. N = 5. RP – red pulp; MZ – marginal zone. * - P<0.05, compared to corresponding mock groups. Inverted triangle – P<0.05, compared to Ly-6G+ cell distribution observed for corresponding compartments in WT mice.