Modified Vaccinia Virus Ankara Triggers Type I IFN Production in Murine Conventional Dendritic Cells via a cGAS/STING-Mediated Cytosolic DNA-Sensing Pathway
Figure 7
Endosomal and lysosomal acidification and lysosomal enzyme activities are required for type I IFN production by BMDCs in response to MVA infection.
(A) GM-CSF-BMDCs (1×106) were infected with MVA at a MOI of 10 for 1 h. Cells were washed and incubated in fresh medium in the presence or absence of chloroquine (50 µM), bafilomycin A1 (100 nM), or CA-074-Me (10 µM). Supernatants were collected 22 h later. The concentrations of IFN-α/β were determined by ELISA. Data are means ± SEM (n = 6). The combined results of three independently performed experiments are shown. **, p<0.01; ***, p<0.001; compared with no drug treatment. (B) GM-CSF-BMDCs were generated from Cathepsin B KO (CathB−/−) and age-matched WT controls (CathB+/+). Cells (1×106) were infected with MVA at a MOI of 10. Supernatants were collected 22 h later. The concentrations of IFN-α/β were determined by ELISA. Data are means ± SEM (n = 3). A representative experiment is shown, repeated twice. **, p<0.01; comparisons were made between CathB+/+ and CathB−/− cells as indicated.