Competitive and Cooperative Interactions Mediate RNA Transfer from Herpesvirus Saimiri ORF57 to the Mammalian Export Adaptor ALYREF
Figure 3
Comparison of ALYREF54–155-ORF57103–120 with ALYREF54–155-ICP27103–138 and U2AF complex structures.
(A) Overlay of the RRM domains of ALYREF in complex with ICP27103–138 (green and magenta, PDB code 2kt5 [30]) and ORF57-bound ALYREF determined here (cyan and orange), demonstrating the shift in α-helix 1 position. (B) ALYREF54–155 in complex with ORF57103–120, determined here. (C) Backbone amide weighted chemical shift changes (δCS) in the ALYREF54–155-ORF57103–120 complex are emphasized by color. δCS>0.3 are red, 0.15–0.299 orange, 0.05–0.149 yellow, prolines are blue and regions unaffected are green. (D) U2AF35 in complex with U2AF65 (PDB code 1jmt, [50]). (E) ALYREF54–155 in complex with ICP27103–138, previously determined (pdb 2kt5). (F) Backbone amide weighted chemical shift changes (δCS) in the ALYREF54–155-ICP27103–120 complex with the same coloring as panel C.